Rowena G Matthews Collegiate Professor in the Life Sciences
Research Professor
Life Sciences Institute
Professor of Cell and Developmental Biology
[email protected]
Available to mentor
Melanie D Ohi PhD
Research Professor
The Ohi lab is interested in understanding how dynamic protein and protein-nucleic acid complexes organize and function in cells. We use a combination of single particle cryo-electron microscopy, biochemistry, and molecular biology approaches to determine 3D structures of biologically important complexes. We work on pore-forming complexes, bacterial secretion systems, membrane sculpting proteins, and protein-nucleic acid complexes.
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Center MemberRogel Cancer Center
-structural biology
-cryo-electron microscopy
-host-pathogen interactions
-membrane proteins
-protein-nucleic acid complexes
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Connolly SM, Erwin AL, Sabb M, Hanks JL, Chang L, Torrez RM, Caso GC, Campbell AM, Mosalaganti S, Cover TL, Ohi MD. J Mol Biol, 2023 Dec 30; 436 (4): 168432Journal ArticleStructural Analysis of Membrane-associated Forms of Helicobacter pylori VacA Toxin.
DOI:10.1016/j.jmb.2023.168432 PMID: 38161000 -
Mullins EA, Salay LE, Durie CL, Bradley NP, Jackman JE, Ohi MD, Chazin WJ, Eichman BF. 2023 Sep 28;PreprintA mechanistic model of primer synthesis from catalytic structures of DNA polymerase α-primase.
DOI:10.1101/2023.03.16.533013 PMID: 36993335 -
Frick-Cheng AE, Shea AE, Roberts JR, Smith SN, Ohi MD, Mobley HLT. bioRxiv, 2023 Sep 28;Journal ArticleAltered motility in response to iron-limitation is regulated by lpdA in uropathogenic E. coli CFT073.
DOI:10.1101/2023.09.27.559868 PMID: 37808639 -
Roberts J, Frick-Cheng A, Durie C, Styron H, Ohi M. Microsc Microanal, 2023 Jul 22; 29 (Supplement_1): 923 - 924.Journal ArticleCharacterization of Two New Proteins Found in the L. pneumophila Dot/Icm T4SS.
DOI:10.1093/micmic/ozad067.457 PMID: 37613758 -
Chauvier A, Porta JC, Deb I, Ellinger E, Meze K, Frank AT, Ohi MD, Walter NG. Nat Struct Mol Biol, 2023 Jul; 30 (7): 902 - 913.Journal ArticleStructural basis for control of bacterial RNA polymerase pausing by a riboswitch and its ligand.
DOI:10.1038/s41594-023-01002-x PMID: 37264140 -
Torrez RM, Nagaraja S, Menon A, Chang L, Ohi MD, Garner AL. Biochemistry, 2023 Jun 6; 62 (11): 1725 - 1734.Journal ArticleComparative Biochemical Studies of Disease-Associated Human Dicer Mutations on Processing of a Pre-microRNA and snoRNA.
DOI:10.1021/acs.biochem.2c00687 PMID: 37130292 -
Han B, Gulsevin A, Connolly S, Wang T, Meyer B, Porta J, Tiwari A, Deng A, Chang L, Peskova Y, Mchaourab HS, Karakas E, Ohi MD, Meiler J, Kenworthy AK. J Biol Chem, 2023 Apr; 299 (4): 104574Journal ArticleStructural analysis of the P132L disease mutation in caveolin-1 reveals its role in the assembly of oligomeric complexes.
DOI:10.1016/j.jbc.2023.104574 PMID: 36870682 -
Torrez RM, Ohi MD, Garner AL. Biochemistry, 2023 Jan 3; 62 (1): 1 - 16.Journal ArticleStructural Insights into the Advances and Mechanistic Understanding of Human Dicer.
DOI:10.1021/acs.biochem.2c00570 PMID: 36534787
Department News
"Structural Analysis of Membrane-associated Forms of Helicobacter pylori VacA Toxin," has been published in the Journal of Molecular Biology
