Ryan E Mills, PhD
Program Director of DCMB Computing Infrastructure
Professor of Computational Medicine and Bioinformatics
Professor of Human Genetics
Computational Medicine and Bioinformatics
100 Washtenaw Ave, Room 2055B
Ann Arbor, MI 48019
[email protected]

Available to mentor

Ryan E Mills, PhD
Professor
  • Qualifications
  • Center Memberships
  • Research Overview
  • Links
  • Recent Publications
  • Qualifications
    • PhD
      Georgia Institute of Technology, Atlanta, 2006
    • MS
      Georgia Institute of Technology, Atlanta, 2003
    • AB
      Wabash College, Crawfordsville, 2000
    Center Memberships
    • Center Member
      Precision Health Initiative
    Research Overview

    The primary purpose of sequencing genomes is to identify the underlying genetic variation between individuals and to explore what role those changes have on human phenotypes. Our research laboratory develops and implements methods to precisely identify and resolve different types of genomic variation both between and within individuals. Our current efforts are focused on developing methods for discovering and characterizing somatic genomic variation in individual cells and tissues. Our goal is to integrate this information with other forms of biologically and medically relevant data to improve our overall understanding of human health and disease.

    Over the past decade, our research has been conducted independently, collaboratively through partnerships with other research groups, and through membership in numerous large consortia including the 1000 Genomes Project, Human Genome Structural Variation Consortium, Brain Somatic Mosaicism Network, Impact of Genomic Variation on Function Consortium, and recently the Somatic Mosaicism across Human Tissues Network.

    Areas of Investigation

    1. Discovery and Analysis of Structural Genomic Variation in Human Populations: Our interest in structural variation began with early work constructing some of the earliest maps of insertion/deletion variation as well as the first large-scale sequence-based assessment of copy number variation across multiple human populations. Our current research has continued in this area, where we have published several methods for identifying different types of structural variation from whole genome sequencing including copy number variation, complex rearrangements, nuclear mitochondrial insertions, and mobile element insertions. We continue to progress in these areas, with ongoing projects studying allelic heterogeneity in polymorphic mobile element insertions and modeling the potential impact of structural variation on gene expression and chromatin structure. We are also investigating short tandem repeat expansions in large cohorts of individuals with neurodevelopment and neuropsychiatric disorders and have been developing cyberinfrastructure for low-cost/high-throughput analysis using Amazon Web Services (AWS) and other cloud-based systems.

    2. Technologies to Identify Somatic Structural Variation in Human Tissues: While there are many studies on somatic structural variation in cancers, there are far fewer that explore their prevalence and impact within non-cancerous human tissues. This has been in part due to limitations of technology, limiting investigations to either higher frequency mosaicism or very large events detectable by microscopy or fluorescence in situ hybridization (FISH) based assays. Our group became involved in this area of research through our participation in the Brian Somatic Mosaicism Network (BSMN), a consortium formed to explore different types of genetic variation within human brain tissue. We helped lead initiatives to explore best practices in somatic SNV calling as well as somatic copy number variation from whole genome amplified single cell. In parallel to our BSMN-related research, we have also been exploring the somatic prevalence of nuclear mitochondrial insertions in the aging human brain. This work in aggregate, coupled with other ongoing research into somatic mobile element insertions, led to a recent award as part of the new Somatic Mosaicism across Human Tissues (SMaHT) consortium to continue to explore this type of variation in other human tissues by developing new tools and technologies.

    3. Characterization and Impact of Human Papilloma Virus (HPV) in Head and Neck Cancers: Our interest in structural variation has further led to an ongoing collaboration on HPV integrations in head and neck squamous cell carcinomas (HNSCC). When HPV integrates into a human genome, it is often associated with structural rearrangements around the insertion site which can result in a focal amplification of the region, resulting in sometimes over 100 copies of the HPV element and surround area. This is likely due to a rolling circle amplification of the HPV genome, and while such amplifications are not present in every HPV-associated HNSCC, we hypothesize that different integration structures are associated with distinct mechanisms for carcinogenesis and progression. Our group developed an approach, SearcHPV, to identify HPV integrations and resulting rearranged genomic structures from targeted capture approaches and have contributed in its application to explore HPV characteristics as a biomarker and its role in mucoepidermoid carcinoma.

    Links
    • Mills Lab
    Recent Publications See All Publications
    • Journal Article
      Impact and characterization of serial structural variations across humans and great apes.
      Höps W, Rausch T, Jendrusch M, Human Genome Structural Variation Consortium (HGSVC) , Korbel JO, Sedlazeck FJ. Nat Commun, 2024 Sep 13; 15 (1): 8007 DOI:10.1038/s41467-024-52027-9
      PMID: 39266513
    • Journal Article
      Mapping recurrent mosaic copy number variation in human neurons.
      Sun C, Kathuria K, Emery SB, Kim B, Burbulis IE, Shin JH, Brain Somatic Mosaicism Network , Weinberger DR, Moran JV, Kidd JM, Mills RE, McConnell MJ. Nat Commun, 2024 May 17; 15 (1): 4220 DOI:10.1038/s41467-024-48392-0
      PMID: 38760338
    • Preprint
      Complex genetic variation in nearly complete human genomes.
      Logsdon GA, Ebert P, Audano PA, Loftus M, Porubsky D, Ebler J, Yilmaz F, Hallast P, Prodanov T, Yoo D, Paisie CA, Harvey WT, Zhao X, Martino GV, Henglin M, Munson KM, Rabbani K, Chin C-S, Gu B, Ashraf H, Austine-Orimoloye O, Balachandran P, Bonder MJ, Cheng H, Chong Z, Crabtree J, Gerstein M, Guethlein LA, Hasenfeld P, Hickey G, Hoekzema K, Hunt SE, Jensen M, Jiang Y, Koren S, Kwon Y, Li C, Li H, Li J, Norman PJ, Oshima KK, Paten B, Phillippy AM, Pollock NR, Rausch T, Rautiainen M, Scholz S, Song Y, Söylev A, Sulovari A, Surapaneni L, Tsapalou V, Zhou W, Zhou Y, Zhu Q, Zody MC, Mills RE, Devine SE, Shi X, Talkowski ME, Chaisson MJP, Dilthey AT, Konkel MK, Korbel JO, Lee C, Beck CR, Eichler EE, Marschall T. 2024 Sep 25; DOI:10.1101/2024.09.24.614721
      PMID: 39372794
    • Journal Article
      Somatic nuclear mitochondrial DNA insertions are prevalent in the human brain and accumulate over time in fibroblasts.
      Zhou W, Karan KR, Gu W, Klein H-U, Sturm G, De Jager PL, Bennett DA, Hirano M, Picard M, Mills RE. PLoS Biol, 2024 Aug; 22 (8): e3002723 DOI:10.1371/journal.pbio.3002723
      PMID: 39172952
    • Journal Article
      Genomic data resources of the Brain Somatic Mosaicism Network for neuropsychiatric diseases.
      Garrison MA, Jang Y, Bae T, Cherskov A, Emery SB, Fasching L, Jones A, Moldovan JB, Molitor C, Pochareddy S, Peters MA, Shin JH, Wang Y, Yang X, Akbarian S, Chess A, Gage FH, Gleeson JG, Kidd JM, McConnell M, Mills RE, Moran JV, Park PJ, Sestan N, Urban AE, Vaccarino FM, Walsh CA, Weinberger DR, Wheelan SJ, Abyzov A, BSMN Consortium . Sci Data, 2023 Nov 20; 10 (1): 813 DOI:10.1038/s41597-023-02645-7
      PMID: 37985666
    • Journal Article
      Author Correction: Machine learning reveals bilateral distribution of somatic L1 insertions in human neurons and glia.
      Zhu X, Zhou B, Pattni R, Gleason K, Tan C, Kalinowski A, Sloan S, Fiston-Lavier A-S, Mariani J, Petrov D, Barres BA, Duncan L, Abyzov A, Vogel H, Brain Somatic Mosaicism Network , Moran JV, Vaccarino FM, Tamminga CA, Levinson DF, Urban AE. Nat Neurosci, 2023 Oct; 26 (10): 1833 DOI:10.1038/s41593-023-01438-w
      PMID: 37648813
    • Journal Article
      Author Correction: The landscape of somatic mutation in cerebral cortex of autistic and neurotypical individuals revealed by ultra-deep whole-genome sequencing.
      Rodin RE, Dou Y, Kwon M, Sherman MA, D'Gama AM, Doan RN, Rento LM, Girskis KM, Bohrson CL, Kim SN, Nadig A, Luquette LJ, Gulhan DC, Brain Somatic Mosaicism Network , Park PJ, Walsh CA. Nat Neurosci, 2023 Oct; 26 (10): 1833 DOI:10.1038/s41593-023-01437-x
      PMID: 37644260
    • Journal Article
      Schizophrenia-associated somatic copy-number variants from 12,834 cases reveal recurrent NRXN1 and ABCB11 disruptions.
      Maury EA, Sherman MA, Genovese G, Gilgenast TG, Kamath T, Burris SJ, Rajarajan P, Flaherty E, Akbarian S, Chess A, McCarroll SA, Loh P-R, Phillips-Cremins JE, Brennand KJ, Macosko EZ, Walters JTR, O'Donovan M, Sullivan P, Psychiatric Genomic Consortium Schizophrenia and CNV workgroup , Brain Somatic Mosaicism Network , Sebat J, Lee EA, Walsh CA. Cell Genom, 2023 Aug 9; 3 (8): 100356 DOI:10.1016/j.xgen.2023.100356
      PMID: 37601975
    Featured News & Stories
    Department News
    Highlighted Publications - August 2024
    Department of Human Genetics faculty are involved in widely diverse areas of research, from Genome Structure and Function research to Evolutionary and Population Genetics.
    Photos of DCMB promoted faculty: Yuanfang Guan, Ph.D., Jie Liu, Ph.D., Ryan Mills, Ph.D., Stephen C.J. Parker, Ph.D., and Arvind Rao, Ph.D.
    Department News
    Five DCMB faculty are promoted, congratulations!
    Five DCMB faculty were promoted. They are Yuanfang Guan, Ph.D., Jie Liu, Ph.D., Ryan Mills, Ph.D., Stephen C.J. Parker, Ph.D., and Arvind Rao, Ph.D. Congratulations!
    Department News
    Highlighted Publications - May 2024
    Department of Human Genetics faculty are involved in widely diverse areas of research, from Genome Structure and Function research to Evolutionary and Population Genetics.
    Department News
    Improving and scaling techniques in both molecular and computational labs with NIH support
    Improving and scaling techniques in both molecular and computational labs with NIH support