Yifan Wang, DVM, PhD
Department of Microbiology and Immunology
1150 West Medical Center Drive, 6706A Medical Science Building II
Ann Arbor, Michigan 48109
[email protected]

Available to mentor

Yifan Wang, DVM, PhD
Assistant Professor
  • About
  • Links
  • Qualifications
  • Research Overview
  • Recent Publications
  • About

    Toxoplasma gondii is a highly successful intracellular protozoan parasite that can infect almost all warm-blooded animals and invade all the nucleated cells. Infection with this parasite can cause severe disease in immunocompromised individuals (e.g., HIV/AIDs patients) and fetuses. Given the difference in the genetic background across various hosts, susceptibility to toxoplasmosis and immune response to the parasite varies widely between humans and other animals. In addition, Toxoplasma is genetically tractable and easy to obtain and cultivate in vitro and in vivo. These make the parasite ideal for studying host-pathogen interactions and innate immunity in different cell types and host species.

    The Wang lab’s long-term research goals are understanding the innate immune machinery determining Toxoplasma resistance between different hosts and demonstrating the molecular basis of Toxoplasma effector-mediated host immune modulation. Activating such anti-Toxoplasma machinery or inhibiting the immunomodulatory parasite effectors could sensitize the parasite to immune surveillance and lead to a better treatment against Toxoplasma infection in immunocompromised patients. To achieve the goals, Wang lab applies a multifaceted approach, including functional genomics, proteomics, bioinformatics, and other biochemical analysis.

    Links
    • Wang Lab
    Qualifications
    • Postdoctoral Fellow
      University of California, Davis, Pathology, Microbiology & Immunology, 2021
    • PhD
      Huazhong Agricultural University, Wuhan, 2015
    • DVM
      Jilin Agricultural University, Changchun, 2010
    Research Overview

    Host-pathogen interaction, Toxoplasma gondii, Innate immunity

    Recent Publications See All Publications
    • Journal Article
      Host E3 ubiquitin ligase ITCH mediates Toxoplasma gondii effector GRA35-triggered NLRP1 inflammasome activation and cell-autonomous immunity.
      Wang Y, Hollingsworth LR, Sangaré LO, Paredes-Santos TC, Krishnamurthy S, Penn BH, Wu H, Saeij JPJ. mBio, 2024 Mar 13; 15 (3): e0330223 DOI:10.1128/mbio.03302-23
      PMID: 38376248
    • Preprint
      Host E3 ubiquitin ligase ITCH mediates Toxoplasma gondii effector GRA35-triggered NLRP1 inflammasome activation and cell-autonomous immunity.
      Wang Y, Hollingsworth LR, Sangaré LO, Paredes-Santos TC, Krishnamurthy S, Penn BH, Wu H, Saeij JPJ. 2023 Dec 14; DOI:10.1101/2023.12.13.571530
      PMID: 38168400
    • Journal Article
      mSphere of Influence: Systemic decoding gene function in Toxoplasma gondii pathogenesis-CRISPR screens and beyond.
      Wang Y. mSphere, 2023 Aug 24; 8 (4): e0027123 DOI:10.1128/msphere.00271-23
      PMID: 37382434
    • Journal Article
      Genome-wide CRISPR screen identifies genes synthetically lethal with GRA17, a nutrient channel encoding gene in Toxoplasma.
      Paredes-Santos TC, Bitew MA, Swale C, Rodriguez F, Krishnamurthy S, Wang Y, Maru P, Sangaré LO, Saeij JPJ. PLoS Pathog, 2023 Jul; 19 (7): e1011543 DOI:10.1371/journal.ppat.1011543
      PMID: 37498952
    • Journal Article
      CRISPR Screens Identify Toxoplasma Genes That Determine Parasite Fitness in Interferon Gamma-Stimulated Human Cells.
      Krishnamurthy S, Maru P, Wang Y, Bitew MA, Mukhopadhyay D, Yamaryo-Botté Y, Paredes-Santos TC, Sangaré LO, Swale C, Botté CY, Saeij JPJ. mBio, 2023 Apr 25; 14 (2): e0006023 DOI:10.1128/mbio.00060-23
      PMID: 36916910
    • Journal Article
      CRISPR screen to determine the in vivo fitness of Toxoplasma genes.
      Sangaré LO, Wang Y, Arranz-Solís D, Saeij JPJ. STAR Protoc, 2021 Jun 18; 2 (2): 100520 DOI:10.1016/j.xpro.2021.100520
      PMID: 34013213
    • Journal Article
      Genome-wide screens identify Toxoplasma gondii determinants of parasite fitness in IFNγ-activated murine macrophages.
      Wang Y, Sangaré LO, Paredes-Santos TC, Hassan MA, Krishnamurthy S, Furuta AM, Markus BM, Lourido S, Saeij JPJ. Nat Commun, 2020 Oct 16; 11 (1): 5258 DOI:10.1038/s41467-020-18991-8
      PMID: 33067458
    • Chapter
      Toxoplasma Mechanisms for Delivery of Proteins and Uptake of Nutrients Across the Host-Pathogen Interface.
      Wang Y, Sangaré LO, Paredes-Santos TC, Saeij JPJ. Annu Rev Microbiol, 2020 Sep 8; 74: 567 - 586. DOI:10.1146/annurev-micro-011720-122318
      PMID: 32680452
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    M&I welcomes Dr. Yifan Wang, D.V.M, PhD
    M&I welcomes Dr. Yifan Wang, D.V.M, PhD